Fig. 3. Protection against F. columnare in zebrafish reconventionalized with individual or mixed bacterial strains isolated from zebrafish.
a Determination of the level of protection provided by each of the 10 bacterial strains composing the core protective zebrafish microbiota. Bacteria were added individually to the water on hatching day (dose 5.105 cfu/mL). b Level of protection provided by different amount of C. massiliae and Mix9. Mix9 only protected at the highest inoculum doses. Mean survival is represented by a thick horizontal bar with standard deviation. Blue mean bars correspond to larvae not exposed to the pathogen and red mean bars correspond to exposed larvae. Larvae mortality rate was monitored daily and surviving fish were euthanized at day 9 post exposition to the pathogen. Indicated statistics correspond to unpaired, nonparametric Mann–Whitney test. ****p < 0.0001; absence of *: non-significant.