Fig. 6. Pre-exposure to C. massiliae protects larval and adult zebrafish against F. columnare infection.
a Zebrafish larvae were inoculated at 4 dpf with 5.105 cfu/mL of C. massiliae for 48 h before infection at 6 dpf with virulent F. columnare strains. b Survival of adult zebrafish with or without pre-exposure to C. massiliae (2.106 cfu/mL for 48 h) followed by exposure to F. columnareALG (5.106 cfu/mL for 1 h) Mean survival is represented by a thick horizontal bar with standard deviation. For each condition, n = 12 zebrafish larvae or 10 adult. Zebrafish mortality rate was monitored daily and surviving fish were euthanized at day 9 post exposition to the pathogens (9 dpi). Blue bars correspond to larvae not exposed to the pathogen and red mean bars correspond to exposed larvae. Indicated statistics correspond to unpaired, nonparametric Mann–Whitney test. ****p < 0.0001; **p < 0.005; absence of *: non-significant.