Figure 2.

Multicolor lightsheet characterization: (A) Schematic diagram of the optical set-up used for light sheet field measurement. Beam shutters (BS) along with mirror (M), neutral density (ND) filters are respectively used to select and scale light intensity in each arm. The beams are combined using a dichroic mirror (DM), expanded 4-times by beam-expander (BE) to completely fill the back-aperture of cylindrical lens (CL). An objective (O) is placed at the focus of cylindrical lens to generate diffraction-limited multicolor light sheet. (B) The field is directly recorded by selecting the desired light (473 nm/532 nm) using BS and a CCD camera which is scanned ($\mathrm{\Delta }Z=10\mathrm{\mu }$m) about the focus of the objective to record the illumination field intensity. The corresponding 3D and 2D views of the field are shown. The recorded fields are also compared with the computational study. Finally, a dual-color light sheet is also shown. (C) Line intensity plots of the field along X,  Y,  Z are used to determine the actual dimension of the light sheet.