Figure 3.

(A) OVSS system is calibrated with standard fluorescent beads, Nile Red (Ex/Em: 535 nm/570 nm, ) and Yellow Green (Ex/Em: 505 nm/515 nm). Images are captured at 3 degree rotation with an exposure time of 10 ms and an internal camera gain of 15 dB. A image size of 400 $\times$ 400 pixel size is cropped (see Supplementary 3) and processed. Reconstructed volume slices of different planes along with few sectional images (at $\theta =9^{\circ },{35}^{\circ },{51}^{\circ }$ ) are also shown. (B) Line plots (along dotted white lines) of slice at different cross-sections of the volume indicate red and green beads that have a FWHM size of $\approx 10\mathrm{\mu }$m.