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. 2021 Apr 7;11:7616. doi: 10.1038/s41598-021-86951-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zebrafish embryo imaging. (A) Leica confocal images (tiled) of entire Zebrafish with a 10X objective lens. 48 h Zebrafish embryo with muscles and yolk stained with TRITC and BODIPY respectively show the distribution of lipids and muscles. (B) Reconstructed volume images of specific sections (marked I-V) of embryo are also shown. (C) 2D slice images from the reconstructed volume. (D) Images of muscle structure (planes, 26, 30 and 33) and primitive macrophages (planes, 12, 29 and 57) 24 hours post fertilization. (See, Supplementary 7 for details.