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. 2021 Apr 7;11:7616. doi: 10.1038/s41598-021-86951-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zebrafish development at various phases indicating a cross-over from lipid-to-protein dependent metabolism. Intensity plot at 6 h period shows BODIPY dominating over TRITC indicating that muscle is yet not developed. At 12 h, there is a rise in TRITC level but dominated by BODIPY, whereas at 24 h significant amount of BODIPY and increased level of TRITC indicates growth of muscles. 48 h volume data shows significant amount of TRITC indicating developed muscles and localised yolk-sac (labeled by BODIPY). Finally, at 96 h and 120 h, BODIPY is localised and distributed over the prominent muscles.