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. 2021 Apr 7;11:7616. doi: 10.1038/s41598-021-86951-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mean ratio plot for determining the transition from lipid based metabolism to protein based metabolism. The data points are obtained by integrating the ratio of TRITC and BODIPY (representing, muscle and yolk-sac respectively in Zebrafish). A linear fit is used to represent the data. The region to the left of the critical transition time ( $τ_{c}$ ) is labeled as lipid-based metabolism and the region to the right of $τ_{c}$ as protein-based metabolism. The critical transition time occurs in the Pharyngula period (approximately at 50 h after fertilization).