Fig. 4.

TGFβ-15-specific CD8⁺ T-cell clones kill target cells in an HLA-A2-restricted manner and kill cancer cell lines expressing TGFβ. A TGFβ-15-specific CD8⁺ T cells effectively lysed T2 cells pulsed with TGFβ-15. B To control that the TGFβ-15 response was indeed HLA-A2 restricted, it was demonstrated that HLA-A2⁺ target cells but not HLA-A3⁺ target cells pulsed with peptide were lysed. C Stimulation of TGFβ-15-specific clones with HLA-A2⁺ cancer cell lines and K562 cells with subsequent analysis of the expression of CD107a and CD137 on T cells. D Stimulation of T cells with UKE-1 and THP-1 in an ICS. E UKE-1 and THP-1 cells used as target cells in Cr51 release assays with TGFβ-15-specific CD8⁺ T cells as effector cells. F The HLA-A2⁺ and TGFβ-expressing cancer cell lines SET-2 and WM-852 were used as target cells in a Cr51 release assay with TGFβ-15-specific T cells as effector cells. K562-A2 loaded with TGFβ-15 peptide was included as a positive control