Table 2.
Optimization of CTL epitopes with immunological parameters for MHC binding, transporter associated with antigen processing (TAP) affinity, C-terminal cleavage efficiency, combinatorial rank, antigenicity, and non-toxic nature characterization.
| Peptide | MHC | TAP | Cle | Comb | %Rank | Antigenicity | Nature | |
|---|---|---|---|---|---|---|---|---|
| 1 | FVSGNCDVV | 0.49600 | 0.17200 | 0.73303 | 0.66523 | 3.00 | Non-antigenic | Non-Toxin |
| 2 | FGGFNFSQI | 0.16600 | 0.25400 | 0.65415 | 0.30683 | 16.00 | Highly-antigenic (1.27) | Non-Toxin |
| 3 | TLDSKTQSL | 0.48400 | 0.58000 | 0.97582 | 0.71806 | 2.00 | Highly-antigenic (1.06) | Non-Toxin |
| 4 | SIIAYTMSL | 0.77400 | 1.27400 | 0.97302 | 1.02478 | 0.15 | 0.5234 | Non-Toxin |
| 5 | MFVFLVLLP | 0.05000 | 0.39800 | 0.13377 | 0.09005 | 50.00 | 0.4166 | Non-Toxin |
| 6 | FVFLVLLPL | 0.68300 | 1.04400 | 0.97043 | 0.92745 | 0.80 | Highly-antigenic (0.86) | Non-Toxin |
| 7 | FRVQPTESI | 0.04400 | 0.75000 | 0.93979 | 0.27420 | 32.00 | Highly-antigenic (0.93) | Non-Toxin |
| 8 | NYNYLYRLF | 0.02500 | 2.73800 | 0.43657 | 0.19168 | 50.00 | Non-antigenic | Non-Toxin |
| 9 | LTDEMIAQY | 0.08000 | 2.65200 | 0.97316 | 0.36526 | 9.00 | Non-antigenic | Non-Toxin |
| 10 | WTAGAAAYY | 0.09900 | 2.83000 | 0.94703 | 0.38283 | 9.00 | Highly-antigenic (0.630) | Non-Toxin |