Figure 3.

EGCG induces a boost of H₂O₂ in L. infantum promastigotes that leads to parasite death. Promastigotes of L. infantum were cultivated in Schneider’s Drosophila medium at 26°C for 72 h with EGCG (125–500 µM) in the absence or in the presence of PEG-catalase (500 U/ml). H₂O₂ was measured with Amplex Red and expressed as the H₂O₂ concentration (A). Cellular viability was measured using the alamar Blue assay (B). Values represent mean ± standard error of three different experiments. ** indicates a significant difference relative to the control group (p < 0.01); *** indicates a significant difference relative to the control group (p < 0.001); # indicates a significant difference relative to the L. infantum incubated with 500 µM of EGCG (p < 0.05); ## indicates a significant difference relative to the L. infantum incubated with 500 µM of EGCG (p < 0.01); ### indicates a significant difference relative to the L. infantum incubated with 500 µM of EGCG (p < 0.001).