Table 2.
Kinetic parameters of L. infantum recombinant trypanothione reductase (TR) in the presence of EGCG.
| EGCG (µM) | Kinetic parameters | |
|---|---|---|
| KM (µM) | Vmax (nmoles 2TNB x mg-1 x min-1) | |
| 0 | 36.4 ± 10.0 | 0.11 ± 0.02 |
| 100 | 60.8 ± 15.9 | 0.12 ± 0.02 |
| 200 | 66.4 ± 18.5 | 0.12 ± 0.02 |
Values represent means ± standard errors of at least four independent experiments performed in triplicate. The KM and Vmax of trypanothione were calculated using a computerized nonlinear regression fit of the data to the Michaelis–Menten equation.