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. 2021 Mar 25;11:675442. doi: 10.3389/fonc.2021.675442

Figure 5.

Figure 5

Circ_0092314 binds to miR-671 and suppresses its expression in PAAD cells. (A) qRT-PCR analysis of miR-671 in PAAD cells following overexpression or knockdown of miR-671. (B) The activities of circ_0092314 reporter containing wild-type (WT) or mutant (MUT) binding sites were determined using luciferase assay following co-transfection with miR-671 mimic or miR-671 inhibitor. (C) RIP assay was performed in PaCa-2 or AsPC-1 cells. The levels of circ_0092314 and miR-671 were detected by qRT-PCR assay. (D) The expression of miR-671 in PAAD cells following knockdown or overexpression of circ_0092314. (E) Cell invasion (left) and sphere formation (right) assay in PaCa-2 cells transfected with (or without) circ_0092314 siRNA, along with (or without) miR-671 inhibitor. (F) Cell invasion (left) and sphere formation (right) assay in AsPC-1 cells transfected with (or without) circ_0092314 vector, along with (or without) miR-671 mimic. ***P < 0.001.