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. 2021 Feb 27;296:100482. doi: 10.1016/j.jbc.2021.100482

Figure 1.

Figure 1

Conditional knockouts of Exoc5 in mouse skeletal muscle were generated.A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, ∗p ≤ 0.05, ∗∗p ≤ 0.01.