Figure 2.

Acute liver-specific deletion of Mogat1 does not improve glucose or insulin tolerance in HFD-fed mice. Male Mogat1 fl/fl mice were fed an HFD starting at 8 weeks of age. After 16 weeks, mice were given a retro-orbital injection of AAV8-TBG-GFP or Cre recombinase (2 × 10¹¹ GC per mouse) and remained on diet for an additional 3 weeks. Mice were fasted for 4 h prior to sacrifice and tissue collection. A: Acute liver-specific Mogat1 knockout did not affect body weight. B,C: Mogat1 knockout did not improve glucose or insulin tolerance. D: Mogat1 knockout reduced Mogat1 gene expression in liver. E: Liver TAG content was not different among groups. G: Hepatic MGAT activity was increased by acute Mogat1 knockdown in liver. Mogat1 knockout did not increase Mogat2, Dgat1, or Dgat2 gene expression in liver. Data are expressed as means ± S.E.M., and two-tailed Student's t-tests were performed, #p < 0.05 gene effect, n = 9.