Figure 3.

Whole-body deletion of Mogat1 causes weight gain and insulin intolerance on an HFD. Male wild-type (WT) and littermate Mogat1 whole-body knockout (MOKO) mice were fed an LFD or an HFD starting at 8 weeks of age for 16 weeks. Mice were fasted for 4 h prior to sacrifice and tissue collection. A: Mogat1 knockout mice gain (MOKO) more weight on an HFD than littermate WT controls. B: ECHO MRI indicates MOKO mice have increased whole body mass, while compared to WT controls. C: Glucose tolerance (1 g/kg lean mass) was not significantly changed in MOKO mice. D,E: HFD-fed MOKO mice had significantly impaired insulin tolerance (1.5 U/kg lean mass) and plasma insulin levels compared to WT controls. F: Mogat1 gene expression was not detectable in MOKO mice on either diet. G: Liver TAG was increased by HFD but unaffected by genotype. H: MGAT activity was unaffected by either diet or genotype. Data are expressed as means ± S.E.M, and two-way ANOVA with Sidak's or Tukey's multiple comparison tests were performed where appropriate, #p < 0.05 gene effect, †p < 0.05 diet effect. For the ITT, two-tailed Student's t-test were performed, #p < 0.05 gene effect n = 6–7.