Figure 4.

Hepatic Mogat1 overexpression increases liver TAG and MGAT activity in mice fed an LFD. Male C57BL6/J mice were fed an LFD at 8 weeks of age. After 6 weeks of diet mice were injected (retro-orbital) with AAV8-TBG-GFP- or AAV8-TBG-Mogat1 (2 × 10¹¹ GC per mouse) and remained on the diet for an additional 10 weeks. Mice were fasted for 4 h prior to sacrifice and tissue collection. A: AAV8-Mogat1-OE did not affect body weight. B,C: Mogat1 overexpression did not impair glucose or insulin tolerance. D: Mogat1 gene expression was significantly increased in AAV8-Mogat1-OE treated mice. E,F: Mogat1 overexpression increased both liver TAG and MGAT activity. G: H&E staining of liver sections reveals similar histology between groups. Data are expressed as means ± S.E.M., and two-tailed Student's t-test were performed, #p < 0.05 gene effect, n = 8–10.