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. 2021 Mar 25;9:647103. doi: 10.3389/fcell.2021.647103

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Copyright © 2021 Zhao, Sun, Xu, Li, Lei, Li, Liu, Ouyang, Sun and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Depletion of FBXO34 impaired the resumption of meiosis and activity of MPF. (A) GVBD rates of control group and FBXO34-MO group at 3 h following release from IBMX inhibition. (B) Western blot of FBXO34-MYC and actin in control, FBXO34 mRNA–injected, and FBXO34-sm mRNA–injected oocytes (100 oocytes per sample). The molecular weight of FBXO34-MYC is 130 kDa; the molecular weight of actin is 42 kDa. (C) After oocytes were released from IBMX inhibition, GVBD rates of oocytes at 1, 2, and 3 h for control group; FBXO34-MO group; and FBXO34-MO+FBXO34-sm mRNA group were summarized. (D) After oocytes were released from IBMX inhibition, Western blot of the phosphorylation level of Tyr15 of Cdk1 (pCdk1-Y15), CCNB1, and actin in the control group and the FBXO34-MO group were presented (180 oocytes per sample). The molecular weight of pCDK1-Y15 is 30 kDa, the molecular weight of CCNB1 is 55 kDa, and the molecular weight of actin is 42 kDa. The relative intensity of immunoreactive bands was quantified by densitometry. Quantitative data were obtained from at least three independent repeats, and one repeat of each experiment contained at least 50 oocytes. The error bars of quantitative data represent the standard deviation (*P < 0.05, ***P < 0.005).