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. 2021 Mar 25;9:647103. doi: 10.3389/fcell.2021.647103

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Copyright © 2021 Zhao, Sun, Xu, Li, Lei, Li, Liu, Ouyang, Sun and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of FBXO34 caused continuous activation of SAC and MI arrest. (A) Control and FBXO34-overexpressed oocytes injected with MAP4-eGFP and H2B-mCherry mRNAs were incubated for 4 h and released to M2 medium for live-cell imaging. The spindle was labeled with MAP4-eGFP, and the chromosomes were labeled with H2B-mCherry. Images of live cells were taken every 30 min from the start of releasing to the end of 14 h later. Scale bar, 20 μm. (B) After being incubated for 9.5 h, control and FBXO34-overexpressed oocytes were collected for chromosome spreading. Bub3 (red), DNA (blue). Scale bar, 10 μm.