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. 2021 Mar 25;9:655440. doi: 10.3389/fcell.2021.655440

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Copyright © 2021 Yang, Li, Tian, Fu, Gao, Zhao, Cao, Liao, Yuan, Liu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The physicochemical, biocompatibility, and immunogenicity properties of DCB and DCB/ECM scaffolds. (A) Macroscopic features and SEM of DCB and DCB/ECM scaffolds. (B) TGF-β3 release kinetics of the DCB/ECM/TGF-β3 scaffold. Values are presented as the means ± SDs (n = 3). (C) Compression modulus of DCB and DCB/ECM (scaffolds; values are presented as the means ± SD, n = 5). (D) SEM of DCB and DCB/ECM scaffolds on which IPFSCs were seeded for 1, 4, and 7 days. (E) Live/dead staining analysis of IPFSCs cultured in DCB and DCB/ECM scaffolds for 7 days. Representative 3D reconstruction images show live (green) cells and dead (red) cells. Values are presented as the means ± SDs (n = 3). (*p < 0.05, n.s. represents no significant difference). (F) Macroscopic observations and H&E staining of the immune responses of DCB and DCB/ECM scaffolds at 1-week postimplantation in rats.