Table 1.
Primer list, including hypothalamic genes; GnRH-I, Gonadotrophin inhibitory hormone (GnIH), OPN5, and VA-Opsin, and pituitary genes; FSH beta-subunit, LH beta-subunit, GnRH-RI, GnRH-RIII, and GnIH-R, along with reference genes, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (ACTB).
| Gene | Direction | Primer sequence (5'-->3') | Accession no. | Reference |
|---|---|---|---|---|
| Hypothalamus | ||||
| GnRH-I | F1 | CTCCTGTTCACCGCATCT | X69491.1 | Avital-Cohen et al., 2013 |
| R2 | CTGCCCTTCTCCTAGACTTTC | |||
| GnIH | F | CGAGTGCTTATTTGCCCTTTG | AB120325.1 | Ahmed et al., 2014 |
| R | CTCCTGGACACCTTGAGATAGA | |||
| OPN5 | F | CAGACGGTTTGGGTTTGACT | NM_001323223.1 | N/A |
| R | TTCAGTGTCTCACGCACCAA | |||
| VA-Opsin | F | GAAAGAAGGCTTCAGGAGCTGAG | XM_015288991.2 | N/A |
| R | CTGCCCACACATGGGAAGAC | |||
| Pituitary gland | ||||
| FSH beta-subunit | F | CCACGTGGTGCTCAGGATACT | NM_204257 | Avital-Cohen et al., 2013 |
| R | AGGTACATATTTGCTGAACAGATGAGA | |||
| LH beta-subunit | F | GTGTTGGTGCTGATGACCCT | HQ872606.1 | Baxter, 2015 |
| R | ACCGCCACCGTTACGTTTAT | |||
| GnRH-RI | F | AATGTGACGGTGCAGTGGTA | AJ304414 | Sparling, 2016 |
| R | GTGTGCACGTGGAAGAGAAA | |||
| GnRH-RIII | F | ATGTACGCCTCCGCCTTCGT | NM_001012609 | Joseph et al., 2009 |
| R | GCAGGGTGACGGTGTGGAAG | |||
| GnIH-R | F | CACTGATGCTGCTGACAGACTA | AB193127.1 | Maddineni et al., 2008 |
| R | CTCATTGAAGTAGCCGTAGATGAT | |||
| Reference genes | ||||
| GAPDH | F | AATGGGCACGCCATCACTAT | NM_204305.1 | Baxter, 2015 |
| R | AGCTGAGGGAGCTGAGATGA | |||
| ACTB | F | GTATGGAGTCCTGTGGTAT | NM_205518.1 | Shimizu and Bedecarrats, 2006 |
| R | CACATCTGCTGGAAGGTGG | |||
1
Forward primer.
2
Reverse primer.