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. 2021 Mar 25;9:635383. doi: 10.3389/fcell.2021.635383

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Copyright © 2021 Usui and Shinohara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rad9 is insensitive to meiotic DSBs. Rad9 binding to chromatin adjacent to mitotic HO-induced DSBs (A) and to meiotic programmed DSBs at the HIS4-LEU2 locus (B) was tested by ChIP. Formaldehyde-fixed chromatin extracts were prepared at 0 and 1 h after HO DSB induction in G2/M-arrested cells, and at 0 and 4 h after meiotic induction, followed by anti-HA and anti-Rad51 ChIP. Anti-HA- or anti-Rad51-bound DNAs were quantified by real-time PCR. Error bars represent standard deviations obtained from 3 independent cultures. The non-tagged wild-type and HA-RAD9 strains examined were USY99 and USY100 in (A) and NKY1551 and USY2035 in (B), respectively.