Fig. 6. Mutations in fhuA cause co-culture specific adaptation.

A The co-culture evolved E. coli, and ΔfhuA mutant, have improved growth in spent medium relative to the E. coli ancestor. Each E. coli population was grown on spent medium from its respective co-culture S. cerevisiae. E. coli ΔbtuB and ΔfhuA mutants were grown on spent medium from a culture from the co-culture evolved “E7” population of S. cerevisiae. Error bars represent standard error of the mean (SEM). Stars show significant differences between strain growth in ancestor and evolved spent medium using post-hoc t-tests. B The change in lag-time for ancestral, coevolved, mutant and wild-type revertant strains when grown in the presence of the FhuA-targeting antibiotic albomycin. Error bars represent standard deviation (SD).