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. 2021 Apr 7;12(4):376. doi: 10.1038/s41419-021-03621-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The expression levels of MCM8 in osteosarcoma tumor tissues and paracarcinoma tissues were determined by immunohistochemical staining. B The expression levels of MCM8 in hFOB1.19 cell line and osteosarcoma cell lines were determined by qRT-PCR. C The knockdown efficiencies of MCM8 in MNNG/HOS cells were evaluated by qRT-PCR. D The transfection efficiencies of shMCM8 in MNNG/HOS and U-2OS cells were evaluated through observing the fluorescence of GFP. Magnification times: ×200. E The MCM8 expression in osteosarcoma cell lines after transfection was analyzed by qRT-PCR. F The expression of MCM8 protein in osteosarcoma cell lines after transfection was detected by western blot. Results were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.