Fig. 1.

Single-cell RNA sequencing. Single B cells and individual barcoded gel beads are oil encapsulated in droplets containing the products required for RT. Each gel bead is labeled with a unique barcode that is attached to a poly(dT) tail to capture mRNA upon cell lysis, as well as an RNA primer to start the RT process. This ensures that when droplets are combined after RT and the cDNA has been sequenced, each sequence can be traced back to the original B cell, allowing native VH-VL pairings to be conserved