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. 2020 Dec 7;28(4):1398–1417. doi: 10.1038/s41418-020-00669-9

Fig. 3. Spontaneous ablation of Pkm2 inhibits cardiac oxidative stress.

Fig. 3

A Higher NADPH/NADP+ ratios in LV extracts obtained from Pkm2KOi mice at 4-d post MI, when compared to Veh-controls. Data are means ± s.e.m. n = 4. *P < 0.001 vs. sham/Veh-control. #P < 0.05 vs. sham/Pkm2KOi. B Expression levels of selected factors that play an important role in detoxification processes of reactive oxygen species (ROS) analyzed by RT-qPCR at 4-d post MI. Data are means ± s.e.m. n = 4. *P < 0.001 vs. sham/Veh-control. #P < 0.001 vs. sham/Pkm2KOi. C Immunoblot analysis of key genes involved in ROS detoxification processes. Western blots were repeated at least once with similar results employing two independent biological replicates. D Levels of 4-HAE, a biomarker for lipid peroxidation, in hearts of Pkm2KOi mice at 4-d post MI. Data are means ± s.e.m. n = 4. *P < 0.001 vs. sham/Veh-control. #P < 0.01 vs. sham/Pkm2KOi. E Levels of GSSG (left) and GSH (right), indicators of cardiac oxidative stress, in Pkm2KOi mice in comparison to MI/Veh-controls at 4-d post MI. Data are means ± s.e.m. n = 4. *P < 0.001 vs. sham/Veh-control. #P < 0.01 vs. sham/Pkm2KOi.