Fig. 3.
Il6 overexpression in pneumocytes triggers neutrophilia in the blood and lungs. Il6-transgenic (Il6tg) and C57BL/6 wild-type (WT) mice were placed in a hypoxic chamber (10% O2) (n = 5 per group) for 3 weeks to induce PH. A The number of neutrophils in the lungs of hypoxic mice was quantified by flow cytometry. B The percentage of neutrophils among DAPI-stained cells and the vasculature remodeling scores of the lungs of hypoxic mice were quantified by confocal microscopy. Arrows indicate lung vasculature-infiltrating neutrophils. C Right ventricular systolic pressure and the Fulton index (RV/LV + S mass ratio) were assessed. The number of neutrophils was also assessed in the blood (D) and bone marrow (E) of hypoxic mice by flow cytometry. The data are shown as the mean ± s.e.m. *P < 0.05; **P < 0.01; ****P < 0.001