Table 1.

Primers and probes used in RT-qPCR.

Target Gene	Primers and Probe Sequences	Standard RNA	Accession Number
28S	Probe 5’ (FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA) 3’	HD11 stimulated with LPS	X59733
	Forward 5’-GGCGAAGCCAGAGGAAACT-3’
	Reverse 5’-GACGACCGATTTGCACGTC-3’
IL2	Probe 5’ (FAM)-ACTGAGACCCAGGAGTGCACCCAGC-(TAMRA) 3’	ExCOS-7 IL-2 mRNA	AJ009800
	Forward 5’-TTGGAAAATATCAAGAACAAGATTCATC-3’
	Reverse 5’-TCCCAGGTAACACTGCAGAGTTT-3’
IL6	Probe 5’ (FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-(TAMRA) 3’	ExCOS-7 IL-6 mRNA	AJ309540
	Forward 5’-GCTCGCCGGCTTCGA-3’
	Reverse 5’-GGTAGGTCTGAAAGGCGAACAG-3’
IL10	Probe 5’ (FAM)-CCAACTGCTCAGCTCTGAACTGCTGGAT-(TAMRA) 3’	ExCOS-7 IL-10 mRNA	AJ621614
	Forward 5’-GAAATTAAGGACTATTTTCAATCCAGAGA-3’
	Reverse 5’-ACAGACTGGCAGCCAAAGGT-3’
IL21	Probe 5’ (FAM)-TGCTGCATACACCAGAAAACCCTGGG-(TAMRA) 3’	ExCOS-7 IL-21 mRNA	AM773757
	Forward 5’-AAAAGATGTGGTGAAAGATAAGGATGT-3’
	Reverse 5’-GCTGTGAGCAGGCATCCA-3’
IFNG	Probe 5’ (FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA) 3’	ExCOS-7 IFN-γ mRNA	Y07922
	Forward 5’-GTGAAGAAGGTGAAAGATATCATGGA-3’
	Reverse 5’-GCTTTGCGCTGGATTCTCA-3’