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. 2021 Mar 25;11:628821. doi: 10.3389/fonc.2021.628821

Figure 3.

Figure 3

Kyn facilitated cells proliferation by AhR/ATK signal. (A) The western blotting of AhR and β-actin in Renca/A498 treated with PBS, Kyn (0.5 μM) or CAFs co-culture. (B) The western blotting of phosphorylated Src, total Src, phosphorylated AKT, total AKT and β-actin in Renca/A498 treated with PBS, Kyn (0.5 μM) or Kyn (0.5 μM) combined with PDM2 (1 nM). (C) The relative cells proliferation of Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with PDM2 (1 nM)/Cap (10 nM). (D) The cytotoxicity of Sor to Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with PDM2 (1 nM)/Cap (10 nM). (E) The cytotoxicity of Sun to Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with PDM2 (1 nM)/Cap (10 nM). (F) The relative migrating cells numbers and representative images of Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with PDM2 (1 nM)/Cap (10 nM). (G) Immunofluorescence staining of AhR in tumor tissues from high degree (H-D) and low degree (L-D) malignant renal patients. The scale bar is 50 μm. (H) The correlation analysis of AhR and phosphorylated AKT in tumor tissues from renal patients. Mean ± SEM, n.s, no significant difference, *p < 0.05, **p < 0.01.