Skip to main content
. 2021 Mar 25;11:628821. doi: 10.3389/fonc.2021.628821

Figure 4.

Figure 4

Kyn regulated cells migration and metastasis through AhR/STAT3 signal. (A) The western blotting of phosphorylated STAT3, total STAT3 in Renca/A498 treated with PBS, Kyn (0.5 μM), Kyn (0.5 μM) combined with PDM2 (1 nM). (B) The cytotoxicity of Sor to Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with S1-109 (2 μM). (C) The cytotoxicity of Sun to Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with S1-109 (2 μM). (D) The relative migrating cells numbers and representative images of Renca/A498 treated with Kyn (0.5 μM) or Kyn (0.5 μM) combined with S1-109 (2 μM). (E), Immunofluorescence staining of phosphorylated STAT3 in tumor tissues from high degree (H-D) and low degree (L-D) renal cancer patients. The scale bar is 50 μm. (F) Immunofluorescence staining of phosphorylated STAT3 in tumor tissues from non-metastatic (non-M) and metastatic renal cancer patients. The scale bar is 50 μm. (G) The correlative analysis of AhR and phosphorylated STAT3 expression in tumor tissues from renal patients. Mean ± SEM, n.s, no significant difference; *p < 0.05, **p < 0.01.