Table 2.
Thirteen isolates obtained from rhizospheric soil samples after enrichment, their species identities and corresponding ASVs recovered in metabarcoding.
| Soil | Isolate ID | Identified as | Most similar ASV | ID% | ASV identified as |
|---|---|---|---|---|---|
| S02 | 2 | Phytophthora plurivora | ASV117 | 98.4 | Phytophthora plurivora |
| S02 | 13 | Phytophthora cambivora | ASV6 | 100 | Phytophthora cambivora |
| S03 | 8 | Phytophthora gonapodyides | ASV18 | 100 | Phytophthora gonapodyides |
| S04 | 10 | Phytopythium citrinum | ASV385 | 98.5 | Phytopythium citrinum |
| S09 | 1 | Phytophthora plurivora | ASV117 | 100 | Phytophthora plurivora |
| S29 | 9 | Phytophthora plurivora | ASV117 | 100 | Phytophthora plurivora |
| S29 | 12 | Phytopythium citrinum | ASV385 | 100 | Phytopythium citrinum |
| S30 | 11 | Phytophthora gonapodyides | ASV18 | 99.1 | Phytophthora gonapodyides |
| S46 | 6 | Phytopythium vexans | ASV267 | 100 | Phytopythium vexans |
| S48 | 3 | Phytophthora cambivora | ASV6 | 100 | Phytophthora cambivora |
| S51 | 4 | Phytophthora gonapodyides | ASV18 | 99.6 | Phytophthora gonapodyides |
| S57 | 7 | Phytopythium vexans | ASV286 | 100 | Phytopythium vexans |
| S58 | 5 | Phytopythium vexans | ASV286 | 99.6 | Phytopythium vexans |
For each isolate, the soil sample it was recovered from (S01–S64), the species it was identified as based on the full ITS sequence, and the most similar ASV are given. Percent (ID%) refer to nucleotide similarity between these ASVs and corresponding isolate’s ITS sequence (obtained by Sanger sequencing) over the length of the ASV (approximately 170–270 bp).