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. 2021 Mar 20;24(4):102339. doi: 10.1016/j.isci.2021.102339

Figure 8.

Figure 8

An M55-SIC homolog shows no affinity for clusterin and HRG and does not activate THP1 cells

(A) Lysates of THP1 cells incubated with 5 μg SIC/ml or DRS +/− 2.5% plasma (P) were subjected to SDS-PAGE analysis and Western blotting. Membranes were probed with antibodies against p38 MAPK (p38) and phosphorylated p38 MAPK (p-p38).

(B) Band intensities of the Western blot in B were analyzed.

(C) Release of TNFα from THP1 cells incubated with SIC or DRS in presence and absence of plasma (P). Absorbance detected at 450 nm.

(D) Activation of NF-κB in cells incubated with 5 μg SIC/ml or DRS in presence and absence of 2.5% plasma (P). Absorbance was detected at 655 nm. All data represent mean ± SEM of 3-4 independent experiments, one-way ANOVA, Dunnett's multiple comparison test, with single pooled variance. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.