Fig. 3.

Enhanced PS exposure during NK cell activation is TMEM16F-dependent. a Anti-TMEM16F immunoblot (IB) of total cell lysates from YT-S cells expressing mouse D409G TMEM16F transfected either with control nontargeting siRNAs (siCT) or with siRNAs against mouse TMEM16F (siTMEM16F). β-actin was used as the loading control. b, c Annexin V and PI staining was performed on the cells described in (a) treated or not with ionomycin. Representative dot plots are shown in (b), while the statistics for four independent experiments are shown in (c). d, e Annexin V and PI staining was conducted with YT-S cells expressing GFP alone or in combination with wild-type TMEM16F or the aspartate 408-to-glycine 408 (D408G) TMEM16F mutant that were incubated or not with K562 cells expressing hCD48 or the combination of PMA (100 ng/ml) plus ionomycin (1 μM) (P + I). Representative dot plots are shown in (d), while statistics for three independent experiments are shown in (e). *p < 0.05; **p < 0.01; ***p < 0.001 (two-tailed Student’s t tests). The data are presented as the means ±  s.e.m