Fig. 5.

Loss of surface 2B4 and membrane dissociation of Src kinases during lipid scrambling. a The intensity of the 2B4 and annexin V staining was analyzed on YT-S derivatives treated with ionomycin (10 μM). Representative dot plots are on the left, while the statistics of the mean fluorescent intensity (MFI) of 2B4 in the annexin V^high versus the annexin V^low populations from three independent experiments are depicted on the right. Cellular localization of Fyn (b) and Lck (c) in YT-S derivatives treated or not with ionomycin (10 μM) for 5 min at RT was determined by staining with the relevant antibodies and confocal microscopy. Representative images are shown in the top two panels, while profiles of the fluorescence as measured by ZEN software are shown in the middle two panels, and the statistics for multiple cells are shown in the bottom two panels. The data are from seven pictures for each condition, with 20–50 cells in each picture. The data are representative of two experiments. Scale bar is 10 μm. *p < 0.05; **p < 0.01; ***p < 0.001 (two-tailed Student’s t tests). The data are presented as the means ± s.e.m. The data are related to Fig. S1