Fig. 6.

The loss of inner membrane acidic charges induced by lipid scrambling. a A depiction of the fluorescent probes used in Figs. 6 and 7 is shown. Cellular localization of mRFP-LactC2 (b), mRFP-K-Ras (c) and Nt-Src-eGFP (d) in YT-S derivatives treated or not with ionomycin (10 μM) for 5 min at RT was determined by confocal microscopy. Representative images are shown at the top, while the statistics for multiple cells are shown at the bottom. The data are from 7 (b, c) or 10 (d) pictures for each condition, with 20–50 cells in each picture. The data are representative of two experiments. The scale bar is 10 μm in all the images. NS, not significant; ***p < 0.001 (two-tailed Student’s t tests). Data are means ± s.e.m