Table 1.
Forward mutation frequencies and survival rates of F1 worms after the TMP/UV mutagenesis.
| Strain | Hatching rate (%) | Twitching assay | PCR detection | ||
|---|---|---|---|---|---|
| Mutation frequency (%) | Estimated number of mutations | Mutation frequency (%) | Estimated number of deletions | ||
| N2 | 100 | 0.22 | 10.2 | < 0.05 | 0.00 |
| wrn-1 | 100 | 0.04 | 1.7 | < 0.05 | 0.00 |
| ced-4 | 90 | 0.14 | 6.5 | 0.1 | 1.12 |
| cku-80 | 95 | 0.07 | 3.3 | 0.2 | 2.23 |
| polq-1 | 95 | 0.60 | 28.0 | < 0.05 | 0.00 |
| pcn-1 | 100 | 0.06 | 3.0 | 0.1 | 1.12 |
| atm-1 | 90 | 0.10 | 4.7 | < 0.05 | 0.00 |
| xpc-1 | 20 | 0.23 | 10.6 | 0.05 | 0.56 |
| atm-1;xpc-1 | 40 | 0.09 | 4.2 | 0.025 | 0.28 |
Forward mutation frequencies were calculated by two methods, twitching assay and PCR detection. The estimated numbers of variants were calculated according to the equations described in materials and methods.