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. 2020 Nov 2;28(4):1284–1300. doi: 10.1038/s41418-020-00651-5

Fig. 6. PD-L1 overexpression can promote its nuclear translocation.

Fig. 6

a IHC assay of nPD-L1 staining with two separate anti-PD-L1 clones (28-8; 2B11D11) on the serial histological section of 24 paired NSCLC tissues. Five cases were selected as representative images. And the red arrows indicated positive nPD-L1 staining (Scale bar: 50 μm). b Immunofluorescence staining of nPD-L1 location in H1299 and HCC827 cell lines analysed by confocal microscopy (Scale bar: 5 μm). c PD-L1-overexpressed cells and control cells were subjected to cellular fractionation followed by western blotting for PD-L1. Tubulin and Lamin B1 were the endogenous markers for cytosolic and nuclear proteins. d Immunofluorescence staining of nPD-L1 location in PD-L1-overexpressed cells compared to control cells (Scale bar: 5 μm). e HEK293T and Hela cells were transfected with the Flag-PD-L1 or control plasmids and then subjected to western blot. NE nuclear extract.