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. 2021 Jan 18;42(3):347–360. doi: 10.1038/s41401-020-00583-3

Fig. 10. DL-NBP suppresses high glucose-induced oxidative stress and reverses downregulation of BDNF level in PC12 cells by acting PI3K/Akt signaling pathway.

Fig. 10

a Western blot analysis of Nrf2, HO-1, NQO1 and SOD2 expression. b–e Quantitative analysis of Nrf2, HO-1, NQO1 and SOD2 expression, n = 3. f Western blot analysis of p-Akt, Akt, p-CREB, CREB and BDNF expression. g–i Quantitative analysis of p-Akt, Akt, p-CREB, CREB and BDNF expression, n = 3. j Immunofluorescence staining of Cleaved-caspase-3 in PC12 cells. Scale bar = 50 μm, n = 5. k Western blot analysis of Bcl-2 and Bax expression. l and m Quantitative analysis of Bcl-2 and Bax expression, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 indicates HG group vs. CON group; #P < 0.05, ##P < 0.01, ###P < 0.001 indicates HG+DL-NBP vs. HG group; &P < 0.05, &&P < 0.01, &&&P < 0.001 indicates CON group vs. HG+DL-NBP+LY294002 group; $P < 0.05, $$P < 0.01, $$$P < 0.001 indicates HG+DL-NBP group vs. HG+DL-NBP+LY294002 group.