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. 2021 Apr 7;12(4):372. doi: 10.1038/s41419-021-03627-6

Fig. 3. Kv1.3 channels localise to the mitochondria.

Fig. 3

A Control HEK293 and HEK293Kv1.3 cells stained with Kv1.3 monoclonal primary antibody (green), MitoTracker Red CMXRos (red) and DAPI (blue). HEK293Kv1.3-mCherry cells with Kv1.3-mCherry (green) and immunostaining of mitochondrial cytochrome C oxidase (red). The white line on the merge images correspond to the plotted line profiles. The plotted line profile show the intensity at each point along the line. Scale bars are 25 μm. B Complex IV respiratory assay in HEK293/Kv1.3 and control HEK293 cells (n = 5). C Citrate synthase activity assay in control HEK293 and HEK293/Kv1.3 cells (n = 3). D Optical density (O.D.) from Western blotting of mitochondrial ATP synthase protein in control HEK293 and HEK293/Kv1.3 cells (n = 3). E Maximum intensity images of control HEK293 (left column) and HEK293/Kv1.3 cells (right column) pretreated with 100 nM MitoTracker Red CMXRos (red). Cell nuclei are stained with DAPI (blue). Scale bars represent 50 μm. F Maximum intensity images of control HEK293 (left column) and HEK293/Kv1.3 cells (right column) stained with anti-Complex IV antibody (red). Cell nuclei are stained with DAPI (blue). Scale bars represent 25 μm. Data were mean ± SEM.