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. 2021 Apr 7;12(4):372. doi: 10.1038/s41419-021-03627-6

Fig. 5. Reactive oxygen species link mitochondrial Kv1.3-induced respiration to cellular proliferation.

Fig. 5

A Representative images showing cellular reactive oxygen species (ROS) in control HEK293 (left) and HEK293/Kv1.3 (right) cells stained with 5 μM CellROX deep red reagent (green) and DAPI to highlight cell nuclei (blue). Scale bars represent 25 μm. B ROS levels in control HEK293 cells and HEK293/Kv1.3 cells stained with 5 μM CellROX deep red reagent (n = 30). C ROS levels in control HEK293 and HEK293/Kv1.3 cells stained with 5 μM CellROX deep red reagent with and without 100 μM menadione pretreatment (n = 30). D Percentage viability of control HEK293 and HEK293/Kv1.3 cells with and without 5 μM MitoQ treatment assessed using trypan blue staining (n = 9). E Control HEK293 and HEK293/Kv1.3 cell counts following 3 days of proliferation, normalised to control, with and without 5 μM MitoQ (n = 9). Data were expressed as mean ± SEM and was analysed using either a Student’s t-test or two-Way ANOVA with Tukey’s post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.