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. 2020 Nov 3;28(4):1270–1283. doi: 10.1038/s41418-020-00650-6

Fig. 5. IL-24 deficiency protects mice against pulmonary fibrosis relies on the reduction of M2 macrophages.

Fig. 5

a The schematic diagram for macrophage adoptive transfer studies. IL-4-induced WT M2 BMDMs were adoptively transferred into clodronate liposomes-treated or PBS liposomes-treated WT and IL-24−/− mice through intratracheal injection at day 7 of BLM induction. b Adoptive transfer of WT M2 macrophages into IL-24−/− mice restored their susceptibility to BLM-induced pulmonary fibrosis. Left panel: a representative result for H&E, Sirius red, and Masson staining, the images were taken under ×200 magnifications. Scale bar, 50 μm. Right panel displays the semiquantitative Ashcroft scores relevant to the severity of fibrosis. Error bars represent means ± SEM (n = 5). Statistical analysis was performed using one-way ANOVA. c Western blot results for analysis of collagen I and fibronectin expression in the lung homogenates from WT and IL-24−/− mice following adoptive transfer. Error bars represent means ± SEM (n = 5). Statistical analysis was performed using one-way ANOVA. M2 M2 macrophages, Clo clodronate liposomes, BLM bleomycin, Coll I collagen I, Fib fibronectin, WT wild type.