Fig. 2.

ASFV E199L protein-induced autophagy is a complete process. A and C Vero and HEK-293T cells were transfected with ASFV E199L protein expression plasmids or empty vectors. Cells were harvested at indicated time points (6, 12, 24 and 36 h) and detected with anti-P62 antibody. Blots are representative of the 3 independent experiments. β-actin was used as sample-loading control. B and D Densitometric P62/β-actin ratios from at least 3 independent experiments were shown. Error bars show standard error of the mean (SEM). Significance was analyzed with two-tailed Student’s test. ***P < 0.001. E and F Vero and HEK-293T cells were transfected with ASFV E199L protein expression plasmids or empty vectors for 24 h and then treated with Lyso-Tracker for 2 h. The fusion between the autophagosomes and lysosomes were observed by confocal microscopy with scale bars indicating 10 µm.