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. 2021 Mar 24;21(6):547. doi: 10.3892/etm.2021.9979

Figure 4.

Figure 4

4EBP1 overexpression could reverse the inhibitory effect of arctigenin on the proliferation, migratory and invasive abilities and EMT in MDA-MB-231 and BT549 cells. (A) Transfection efficiency was examined by reverse transcription quantitative PCR and western blotting. (B) Cell proliferation of transfected MDA-MB-231 and BT549 cells treated with arctigenin was detected using Cell Counting-Kit 8 assay. Transwell assay was used to evaluate the (C) migratory ability or (D) invasive ability of MDA-MB-231 and BT549 cells treated with arctigenin and following transfection with pcDNA3.1-NC or pcDNA3.1-4EBP1 (scale bar, 150 µm). (E) Western blotting was used to evaluate the protein expression of E-cadherin, N-cadherin and vimentin in MDA-MB-231 and BT549 cells treated with arctigenin and following transfection with pcDNA3.1-NC or pcDNA3.1-4EBP1. ***P<0.001 vs. pcDNA3.1-NC group, Control group or Arctigenin (40 µM) + pcDNA3.1-NC group.