. 2021 Mar 25;7(3):e06577. doi: 10.1016/j.heliyon.2021.e06577

Table 1.

Body weight gain and relative organ weight of male and female ICR mice after 90-day oral administration of AuNPs.

	Gender	Control	AuNPs			F value	P value	Degree of freedom
	Gender	Control	0.2 mg/kg	2 mg/kg	20 mg/kg	F value	P value	Degree of freedom
Body weight
Initial body weight (g)	Male	33.1 ± 1.3^a	34.0 ± 1.7^a	32.8 ± 1.9^a	34.1 ± 1.3^a	1.42	0.25	37
Initial body weight (g)	Female	26.5 ± 1.0^a	27.3 ± 1.0^a	27.3 ± 2.2^a	27.2 ± 1.0^a	0.69	0.56	39
Final body weight (g)	Male	44.8 ± 3.4^a	44.2 ± 4.1^a	41.1 ± 3.0^a	42.0 ± 4.0^a	2.15	0.11	37
Final body weight (g)	Female	35.0 ± 2.6^a	34.8 ± 2.8^a	35.7 ± 3.6^a	33.4 ± 2.5^a	1.08	0.37	39
Weight gain (g)	Male	11.2 ± 0.6^a	11.1 ± 1.6^a	10.1 ± 1.7^a	10.5 ± 1.9^a	3.10	0.04	37
Weight gain (g)	Female	8.5 ± 2.7^a	7.5 ± 3.0^a	8.4 ± 3.1^a	6.2 ± 2.2^a	1.40	0.26	39
Relative organ weight
Liver (%)	Male	5.05 ± 0.35^a	4.96 ± 0.37^a	5.09 ± 0.24^a	5.10 ± 1.04^a	0.08	0.97	36
Liver (%)	Female	4.54 ± 0.54^ab	4.98 ± 0.57^a	4.46 ± 0.45^b	4.79 ± 0.24^ab	2.56	0.07	39
Kidney (%)	Male	1.67 ± 0.11^a	1.81 ± 0.34^a	1.79 ± 0.18^a	1.67 ± 0.14^a	1.18	0.33	36
Kidney (%)	Female	1.36 ± 0.09^b	1.52 ± 0.20^a	1.44 ± 0.18^ab	1.48 ± 0.14^ab	1.98	0.13	39
Spleen (%)	Male	0.22 ± 0.02^a	0.26 ± 0.04^a	0.28 ± 0.06^a	0.36 ± 0.38^a	0.91	0.45	36
Spleen (%)	Female	0.36 ± 0.08^ab	0.41 ± 0.09^a	0.34 ± 0.06^b	0.34 ± 0.06^ab	2.09	0.12	39

Data were presented as the mean ± SEM (n = 10 per group). Mean values within each column with different labels are significantly different (p < 0.05) according to one-way ANOVA followed by Tukey's post hoc test. The relative organ weight was expressed as a percentage of body weight.