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. 2021 Apr 7;12:2091. doi: 10.1038/s41467-021-22058-7

Fig. 3. Myofibrils mechanically shape flight muscle mitochondria.

Fig. 3

a Live dissection of hemithorax in which actin has been labelled with Cherry-Gma expressed with Mef2-GAL4. Black rectangle indicates a severed area, in which myofibrils have been mechanically cut, magenta rectangle marks an intact area. b–e High magnification confocal sections of intact (b, d) and severed areas (c, e) of unfixed flight muscles in which mitochondria have been labelled with MOM-GFP (expressed with Mef2-GAL4) (b, c) or with mito-GFP together with Cherry-Gma to label myofibrils (d, e). Note the spherical mitochondria shape and their disengagement from the myofibrils in the severed areas. f, g High magnification of unfixed flight muscle from wild type (f) or Mhc[10] mutant (g) adults genetically labelled with Cherry-Gma to label myofibrils (expressed with Mef2-GAL4) (f, g) and mito-GFP to label mitochondria (f′, g′). Note how similar the rounded Mhc[10] mitochondria (g′) are to the ones in severed myofibrils in e′. Scale bars are 100 µm (a) and 5 µm (b–g).