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. 2021 Apr 7;12:2091. doi: 10.1038/s41467-021-22058-7

Fig. 4. Mitochondria dynamics can impact myofibril development.

Fig. 4

a–i Adult hemi-thoraces (a, b, f, g), flight muscles (d, e, h, i) and flight test (c) of the indicated genotypes. Actin has been visualised with phalloidin, mitochondria with mito-GFP. White dashed lines outline the myofibrils in the yz cross-sections (d″, e″). Note the small round mitochondria present between normal myofibrils upon Marf knock-down (e). Marf over-expression using UAS-Marf-1 or UAS-Marf-2 with Mef2-GAL4 causes fibre atrophy (f, g) and cross-striated myofibrils (h, i). Note that the mitochondria are largely separated from the aligned myofibrils, outlined by the dashed white line on the yz cross-sections (h″, i″). j–l Quantification of mitochondrial content (relative to actin area) (j; n = 12, 12, 12, 11 animals, respectively), of individual mitochondrial area in a single confocal section (k; n = 6894 and 14443 total mitochondria, from five animals in each case) and the cross-striation index of the indicated genotypes (l; n = 6, 17, 10 animals, respectively). In all plots the mean ± standard-deviation (SD) is indicated, each dot the value from single animals (j, l) or confocal sections (k), and significance from two-tailed unpaired t-tests is denoted as p-values ***p ≤ 0.001, ****p ≤ 0.0001. n.s. non-significant. Scale bars are 100 µm (a, b, f, g) and 5 µm (d, e, h, i).