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. 2021 Apr 7;12:2091. doi: 10.1038/s41467-021-22058-7

Fig. 7. Developmental effect of mitochondria hyper-fusion.

Fig. 7

a–h Developing wild-type flight muscles at 24 h after puparium formation (APF) (a, b) and 32 h APF (c, d), compared to Mef2::Marf-1 flight muscles at 24 h APF (e, f) and 32 h APF (g, h). See also Supplementary Movie 8. Mitochondria were visualised with mito-GFP and actin with phalloidin. Note the mitochondrial intercalation between myofibrils in wild-type 32 h APF flight muscles (c″), which is blocked by Mef2::Marf-1 (g″). i–l Developing wild-type (i, k) compared to Mef2::Marf-1 (j, l) flight muscles at 24 h APF visualised by electron microscopy (EM). Two representative regions at different magnifications are shown. Note the clustered and more rounded mitochondria upon Mef2::Marf-1. Scale bars are 2.5 µm (a–g) or directly noted on EM images (i–l).