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. 2021 Apr 7;12:2091. doi: 10.1038/s41467-021-22058-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a–h Developing wild-type flight muscles at 32 h after puparium formation (APF) (a, b) and 48 h APF (e, f), compared to Act88F::Marf-1 flight muscles at 32 h APF (c, d) and 48 h APF (g, h). See also Supplementary Movie 9. Mitochondria were visualised by immunostaining against complex V (ATPase) and actin with phalloidin. Note how mitochondria isolate myofibrils in wild type (e″) but fail to do so in Act88F::Marf-1 with mitochondria clustering centrally (g″). Scale bars are 2.5 µm.