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. 2020 Nov 16;46(3):509–518. doi: 10.1038/s41386-020-00904-4

Fig. 1. Effect of chronic intermittent exposure to ethanol vapors on PACAP immunoreactivity in the BNST and in the CeA 8–10 h after vapor offset.

Fig. 1

Effect of chronic intermittent exposure to ethanol vapors on PACAP immunoreactivity in the BNST (A–D) and in the CeA (E–H) 8–10 h after vapor offset. Ethanol-dependent rats showed an enhanced expression of PACAP immunoreactivity in BNST (A), but not CeA (E), compared to control, nondependent rats. Drawing of coronal rat brain slices representing the area analyzed in BNST (B) and CeA (F). Representative pictures of PACAP immunoreactivity in the BNST (C–D) and CeA (G–H) in control nondependent and in ethanol-dependent rats, respectively. STMA BNST medial-anterior, STLP BNST lateral-posterior, STLD BNST lateral-dorsal, STLJ BNST lateral-justacapsular, CeM CeA medial, CeL CeA lateral, CeC CeA capsular. Scale bars represent 250 μm. Control and ethanol-dependent rats are depicted in orange and green, respectively. Data represent Mean ± SEM (n = 10–11/group for BNST, n = 7–11/group for CeA). #p < 0.05 vs. Controls.