No difference was found between groups (A). Drawing of a coronal rat brain slice representing the area analyzed (B). Representative pictures of PACAP immunohistochemistry in control, nondependent and in ethanol-dependent rats (C–D). STMA BNST medial-anterior, STLP BNST lateral-posterior, STLD BNST lateral-dorsal, STLJ BNST lateral-justacapsular. Scale bars represent 250 μm. Control and ethanol-dependent rats are depicted in orange and green, respectively. Data represent Mean ± SEM (n = 9–10/group).