Table 2.
Incubation condition | Average cell-to-cell distance | Producer + receivers (~21%), receivers only (~79%) | Non-producer + receivers (~21%), receivers only (~79%) | ||
---|---|---|---|---|---|
Beads with growth | Average fluorescence of grown cells | Beads with growth | Average fluorescence of grown cells | ||
No diffusion from beads | 15 µm | 14 ± 1% | 80 ± 11% | 16 ± 0% | 17 ± 4% |
Diffusion from beads, no competing glucose consumers | 15 µm | 75 ± 7% | 131 ± 6% | 6 ± 1% | 1.3 ± 0.4% |
Diffusion from beads, competing glucose consumers | 15 µm | 15 ± 3% | −3.5 ± 4.7% | 9 ± 2% | −4.5 ± 2.7% |
0 µm | 3 ± 2% | 39 ± 7% | 4 ± 2% | −0.7 ± 10% |
Summary of the experimental results of Fig. 3. The columns “beads with growth” give the percentage of beads that were gated as “growth” (n = 3, mean ± sd) for co-cultures of producers and receivers (corresponding to the density plots in Fig. 3B1, D1, D3, F1), and for co-cultures of non-producers and receivers. The columns “average fluorescence of grown cells” give the fluorescence of grown cells on a scale of 0–100% (n = 3, mean ± sd). The median of the log(average fluorescence of grown cells) of the producer-control sample was set to 0% and the median of the log(average fluorescence of grown cells) of the receiver-control sample to 100%. A higher percentage indicates more growth of receiver cells. The average fluorescence of grown cells can exceed the value of the receiver-control sample (see for example Fig. 3D2), causing the percentage to be higher than 100%. Corresponding density plots are shown in Fig. 3B2, D2, D4, F2.