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. 2020 Oct 19;15(3):688–701. doi: 10.1038/s41396-020-00806-9

Table 2.

Consortium response in different spatial structures.

Incubation condition Average cell-to-cell distance Producer + receivers (~21%), receivers only (~79%) Non-producer + receivers (~21%), receivers only (~79%)
Beads with growth Average fluorescence of grown cells Beads with growth Average fluorescence of grown cells
No diffusion from beads 15 µm 14 ± 1% 80 ± 11% 16 ± 0% 17 ± 4%
Diffusion from beads, no competing glucose consumers 15 µm 75 ± 7% 131 ± 6% 6 ± 1% 1.3 ± 0.4%
Diffusion from beads, competing glucose consumers 15 µm 15 ± 3% −3.5 ± 4.7% 9 ± 2% −4.5 ± 2.7%
0 µm 3 ± 2% 39 ± 7% 4 ± 2% −0.7 ± 10%

Summary of the experimental results of Fig. 3. The columns “beads with growth” give the percentage of beads that were gated as “growth” (n = 3, mean ± sd) for co-cultures of producers and receivers (corresponding to the density plots in Fig. 3B1, D1, D3, F1), and for co-cultures of non-producers and receivers. The columns “average fluorescence of grown cells” give the fluorescence of grown cells on a scale of 0–100% (n = 3, mean ± sd). The median of the log(average fluorescence of grown cells) of the producer-control sample was set to 0% and the median of the log(average fluorescence of grown cells) of the receiver-control sample to 100%. A higher percentage indicates more growth of receiver cells. The average fluorescence of grown cells can exceed the value of the receiver-control sample (see for example Fig. 3D2), causing the percentage to be higher than 100%. Corresponding density plots are shown in Fig. 3B2, D2, D4, F2.